Ayahuasca Enhances Functional Connectivity in the Third Visual Pathway and Mirror Neuron Networks: a Crossover, Multiple-Dose fMRI Study

This study provides evidence that ayahuasca enhances connectivity in brain networks involved in high-level visual processing and social cognition, linking these neural changes to subjective improvements in perspective-taking and social functioning.

“Understanding the neural mechanisms underlying the impact of psychedelics on social perception and cognition may be instrumental to unravel their therapeutic potential. We conducted a pharmacoimaging study to examine ayahuasca’s effects on a key theory of mind region, at the core of the third visual pathway (TVP) – the posterior superior temporal sulcus (pSTS), which is involved in facial emotion recognition and social perception.

Twelve healthy participants (mean age: 40 ± 6.6 years; 4female) completed a crossover design with three conditions: 0.5 mg/kg DMT, 0.8 mg/kg DMT, and placebo, with 1–2 months washout intervals. Resting-state fMRI was used to assess pSTS functional and effective connectivity.

The highest dose significantly increased right pSTS connectivity and directed modulation from visual (primary and extrastriate cortices) and mirror-neuron regions (supplementary motor cortex; SMC). Subjectively, this enhanced social cognitive states, with a strong positive correlation between pSTS-SMC connectivity and perspective-taking experiences. Additionally, ayahuasca produced positive psychological effects, including improved perceived social relationships, at one-week follow-up despite minimal acute effects.

Our findings reveal a novel mechanism of action of psychedelics at early stages of social information processing, with enhanced integration of the TVP and mirror-neuron systems. The pSTS emerged as a critical hub supported by top-down and bottom-up evidence, providing a basis for understanding ayahuasca’s prosocial therapeutic effects”.

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Carla Soares, Gisela Lima, Marta Teixeira, Rebeca André, Patrícia Rijo, Célia Cabral, Miguel Castelo-Branco, Ayahuasca Enhances Functional Connectivity in the Third Visual Pathway and Mirror Neuron Networks: a Crossover, Multiple-Dose fMRI Study, Social Cognitive and Affective Neuroscience, 2026;, nsag004, Read Paper

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